WebUpon isolation of DPCs by PxP, samples were analysed using western blotting using the indicated antibodies. d HeLa T-REx Flp-In SPRTN-ΔC cells were complemented with YFP, YFP-SPRTN-Strep variants as indicated (wildtype (WT), E112Q (EQ), R185A (ZBD*)) and treated as shown in Fig. , before DPCs were isolated using PxP and analysed by … WebApr 26, 2024 · Flp-In T-REx 293 cells expressing c-IBD-SBP-YAP1C were incubated in DPBS containing 10 mM 3-AT and supplemented or not with 1 mM H 2 O 2. After 10 min, free thiol groups were blocked with NEM, and the samples were processed as depicted in panel A and subsequently subjected to immunoblotting with rabbit pre-immune serum …
How to grow Flp-In Trex 293 cells in suspension? - ResearchGate
WebThe Invitrogen™ Flp-In™ System allows integration and expression of your gene of interest in mammalian cells at a specific genomic location. The Flp-In System involves … WebApr 21, 2024 · Flp-In t -293细胞系包含稳定整合的pFRT lacZeo和pcDNA 6 TR(来自T-REx系统)。 用Flp- in表达载体和Flp重组酶载体pOG44共转染Flp- in细胞系,可将表达载体定向整合到每个细胞的同一位点,保证基因表 … boise id to joplin mo
U2os Flp In T Rex Cells Thermo Fisher Bioz
WebAug 20, 2024 · The Flp-In system integrates a vector into cell lines at a specific genomic locus, but has not been used for integration of more than one gene. ... All experiments … WebFlp-In™ 293 T-REx cell lines is designed for rapid generation of stable cell lines that ensure homogenous expression of your protein of interest from a Flp-In™ expression vector. … Flp-In™ 293 T-REx cell lines is designed for rapid generation of stable cell lines that … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes WebDescription. Once Flp-InT-REx host cell line containing integrated FRT site has been created, subsequent generation of Flp-InT-REx cell lines expressing gene (s) of interest … boise id to grand teton national park