Fluorescent protein paraffin embedding
WebThe pairing of a primary antibody and secondary antibody (specific for the host species of the primary) that is conjugated to a fluorophore is known as indirect staining. The use of primary antibodies conjugated to … WebAug 11, 2024 · Applying this method, we analyzed 20 different proteins in each of ~67,000 cells in a human formalin-fixed paraffin-embedded (FFPE) tonsil tissue. Based on their unique protein expression profiles and microenvironment, these individual cells are partitioned into different cell clusters.
Fluorescent protein paraffin embedding
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WebHeart tissue paraffin sections with a thickness of 5 µm were incubated with 10 μM DHE (D23806, Invitrogen, CA, USA) at 37 °C for 30 min in the dark. Images were acquired with fluorescent microscopy as well as quantification of the fluorescence intensity (red staining) of DHE using Image J software. 2.9. Immunohistochemistry analysis Paraffin embedding is a well-developed method that is widely used as a basic tool for histomorphology. Compared to other methods, … See more All datasets generated for this study are included in the manuscript and/or the Supplementary Files. The raw data supporting the … See more In this study, we explored the mechanism of GFP fluorescence quenching during paraffin embedding and developed an optimized protocol based on a mechanism we have called TBA … See more
WebImmunohistochemical detection using chromogenic and fluorescently labeled antibodies. For the detection of vascular endothelial zinc finger (VEZF) protein paraffin-embedded brain tissue was processed and probed with a rabbit anti-VEZF polyclonal primary antibody. WebParaffin embedding is widely used in microscopic imaging for preparing biological specimens. However, owing to significant fluorescence quenching during the embedding process, it is not...
WebThe Frederick National Laboratory for Cancer Research (FNLCR) Molecular Histopathology Laboratory (MHL) services the NIH communities in Bethesda and Frederick, Maryland. Contact DetailsDirector: Larry Sternberg, PhDPhone: 301.846.6881 Email: [email protected] Address: Building 469, Room 218, Miller Drive, FNLCR, … WebTransgenes that use GFP-based proteins as a reporter provide a convenient way to follow protein expression over the course of development and disease progression in the …
WebJul 28, 2024 · In this protocol, embedding process included three steps. First, we poured the paraffin wax into the mold before embedding and stored the mold for at least 12 h …
WebApr 1, 2024 · Applying this approach, we have demonstrated that multiple proteins are unambiguously detected in the same set of cells, regardless of the protein analysis order. We have also shown that this method can be successfully applied to quantify proteins in formalin-fixed paraffin-embedded (FFPE) tissues. ready or not raid meth locationsWebApr 12, 2024 · IHC can be used in both transmitted light and fluorescence microscopy, and it can be used in combinations to identify and mark multiple proteins of interest. While many IHC techniques have been published, a certain amount of confusion exists as to how to optimize IHC procedures. how to take care of schizophreniaWebThe fixative used is influenced by the target antigen as well as the desired detection technique ( fluorescent or chromogenic ). The tissue sample is then either embedded in paraffin or frozen. Embedding is important in preserving tissue morphology and giving the tissue support during sectioning. ready or not post office mapWebThe Frederick National Laboratory for Cancer Research (FNLCR) Molecular Histopathology Laboratory (MHL) services the NIH communities in Bethesda and Frederick, Maryland. … how to take care of seahorseshow to take care of silestoneWeb1 hour ago · The post-synaptic density protein 95 (PSD95) is a crucial scaffolding protein participating in the organization and regulation of synapses. PSD95 interacts with numerous molecules, including neurotransmitter receptors and ion channels. The functional dysregulation of PSD95 as well as its abundance and localization has been implicated … how to take care of senior catsWebThe most common method uses paraffin embedding. Frozen sections and floating sections are other options – each method has advantages and limitations (Table 1). Sample fixation is key to tissue processing and is critical to prevent the degradation of … how to take care of seedlings