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Klf4 phase separation

WebApr 12, 2024 · Hydrocyclones are widely used in the separation of two-phase immiscible mixtures. In various application scenarios, three-phase hydrocyclones (3PH) are often urgently needed. In this work, the typical 3PHs are classified into six categories according to their structural characteristics. A comprehensive analysis of their structural feature ... WebDec 17, 2024 · Phase separation promotes cell fate transitions, whereas disruption of phase attenuates the transitions. The major challenge is how to effectively predict mutation residues. For complete details on the use and execution of this protocol, please refer to Wang et al. (2024). Graphical abstract Download : Download high-res image (225KB)

New insight into the significance of KLF4 PARylation in …

WebHere we investigate the role of DNA in nucleation of condensates, using the pioneer transcription factor KLF-4. We show that KLF-4 forms liquid-like condensates on the DNA … WebSep 23, 2024 · The study (“ Liquid condensation of reprogramming factor KLF4 with DNA provides a mechanism for chromatin organization ”), published in Nature Communications, shows that the binding of KLF4 can... fhgp63cb https://kathsbooks.com

Quantifying the phase separation property of chromatin …

WebApr 10, 2024 · Liquid‒liquid phase separation (LLPS) facilitates the formation of membraneless biomolecular condensates, which are crucial for many cellular processes in plants, including embryogenesis, the floral transition, photosynthesis, pathogen defense, and stress responses. The main component required for LLPS is a protein harboring key ... WebJan 14, 2024 · Phase separation describes the large-scale, three-dimensional concentration of proteins and nucleic acids ( Box 1) and is a means by which transcriptional machinery and multiple regulatory elements are brought together. WebAug 28, 2013 · KLF4, as a member of KLF family, expresses in a wide range of tissues in mammals, and plays a critical role in regulating a diverse array of cellular processes … fhgp71al

Klf4 forms liquid droplets in vitro a, Different phase separation ...

Category:Dynamical reorganization of the pluripotency transcription

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Klf4 phase separation

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WebFeb 14, 2024 · Phase separation of proteins/nucleic acids to form non-membrane organelles is crucial in cellular gene-expression regulation. However, little is known about … WebFeb 3, 2024 · We show that Klf4 can phase separate on its own at high concentrations, but at low concentrations, Klf4 only forms condensates on DNA. Using optical tweezers, we …

Klf4 phase separation

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WebKLF4 belongs to the Kruppel-like factor (KLF) family, a group of conserved zinc finger TFs that regulates essential biological processes such as proliferation and development. Reflecting pioneer factor activity, KLF4 uses two out of its three zinc fingers to target a GC-rich hexameric motif enriched within nucleosomal targets22 ( Fig. 7.2 C). WebApr 13, 2024 · Many proteins undergo a process of phase separation that leads to the formation of biomolecular condensates. It has been observed that some of these proteins form dense droplets of sizeable dimensions already below their critical concentration for phase separation. To understand this phenomenon, which is not readily compatible with …

WebAug 26, 2024 · Admittedly, phase separation is a young biological research direction, and many problems persist to overcome how to precisely regulate phase separation, such as … WebDec 7, 2024 · KLF4 plays a critical role in determining cell fate responding to various stresses or oncogenic signaling. Here, we demonstrated that KLF4 is tightly regulated by …

WebJun 18, 2024 · KLF4 represses transcription of the EGFR gene, leading to reduced levels of total EGFR, its activated/phosphorylated form (pEGFR), and its downstream signaling intermediates. Moreover, KLF4 suppression of EGFR is a necessary intermediary step for KLF4 to inhibit aggressive TNBC phenotypes.

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WebSep 22, 2024 · KLF4 is thought to induce long-range pluripotency contacts through condensation at promoters of pluripotency factors (Sharma et al. 2024 ). However, KLF4 condensation depends on KLF4-DNA... department of home affairs pinetownWebHier we probe that nature of transcription factor condensates using the pioneer transcription factor Krüppel-like factor 4 (Klf4). We show such Klf4 can phase separate on its own at highly concentrations, but at low concentrations, Klf4 only forms condensates set DNA. department of home affairs pampierstadWebAug 28, 2013 · These studies suggest that KLF4 is a critical factor in regulating entry of the cells into the mitotic phase. Finally, KLF4 was found both necessary and sufficient in preventing centrosome amplification ... Characterization of different KLF4 isoforms and/or separation of distinct KLF4 expressing cells will be necessary for dissecting specific ... department of home affairs registerWebSep 22, 2024 · The study, published in the journal Nature Communications, reveals that the binding of KLF4 can cause DNA to condense into a separate liquid phase in a process … fhgp80cbWebApr 3, 2024 · Gliomas, accounting for ~25% of primary CNS tumors, have an average mortality rate of 4.43 per 100,000 and 16,606 deaths per year [1, 2].Standard therapies of surgery resection combined with ... department of home affairs potchefstroomWebMar 23, 2024 · The evident changes observed in cell morphology together with the downregulation of the naïve pluripotency markers Nanog, Klf4 and Esrrb and the increase of the mRNA levels of primed... department of home affairs scottburghWebSep 22, 2024 · The KLF4 DNA binding domain phase separates with cognate DNA Purified KLF4 DBD is readily soluble and does not condense or precipitate at physiological salt or upon addition of 10% PEG 8000,... Metrics - Liquid condensation of reprogramming factor KLF4 with DNA ... - … fhgpopular now on bing